Physical and chemical parameters of the crystal growth are studied in great detail to have data for the batch process planning. The crystallization method is optimized in combination of the complete downstream process development. The objective is to simplify the overall process and to achieve highest protein purity in combination with low cost and high yield of the product. Crystallization method plays key role in achieving these targets.
In large scale process the product yield is the most important single cost factor. In a well developed process, the protein yield can be very high. Often the crystallization step has the highest yield of the entire process. The purifying power of crystallization is usually better than any other method. Crystallization holds the reputation of ultimate proof of purity in all chemicals.